The Northern Rebellion of 1569, also known as the Revolt of the Northern Earls, was the only major armed rebellion during the reign of Elizabeth I. In the last months of 1569, the earls of Northumberland and Westmorland rebelled against the queen in an attempt to preserve Catholicism. The establishment of the Elizabethan settlement alienated those who favoured the old religion, and their disaffection increased as growing numbers were arrested and imprisoned for religious nonconformity. This disaffection was spurred by the arrival in England of Mary, Queen of Scots, in 1568, the year before the rebellion. Mary had been forced from her throne after the murder of her second husband Henry, Lord Darnley, and her swift remarriage to Darnley’s suspected murderer, James, Earl of Bothwell. Mary’s Catholic faith made her a sympathetic figure to traditionalists in England. Although the majority of English Catholics remained loyal to Elizabeth, some were determined to force her from the throne and replace her with her cousin Mary, who they hoped would restore Catholicism to the realm.