Polydore Plasden was born in 1563 and was the son of a London horner. He was educated at Rheims and then at the English College in Rome. He was ordained as a priest on 7th December 1586. He remained in Rome for a year after his ordination and then moved back to Rheims, where he stayed from 8th April to 2nd September 1588.

When Polydore returned to England in 1588, he ministered in Sussex and London until 1591, at which point he was captured on 2nd November. He was captured in London at Swithun Wells’ house, where Edmund Gennings was celebrating Mass alongside other fellow Catholics who were captured beside Polydore.